diff --git a/extensions/notebook/src/jupyter/jupyterServerInstallation.ts b/extensions/notebook/src/jupyter/jupyterServerInstallation.ts
index adc1f928e4..fd3cb5e578 100644
--- a/extensions/notebook/src/jupyter/jupyterServerInstallation.ts
+++ b/extensions/notebook/src/jupyter/jupyterServerInstallation.ts
@@ -333,11 +333,6 @@ export class JupyterServerInstallation implements IJupyterServerInstallation {
 		delete env['Path']; // Delete extra 'Path' variable for Windows, just in case.
 		env['PATH'] = this.pythonEnvVarPath;
 
-		// We don't want Jupyter to know about DOTNET_ROOT, as it's been modified by the liveshare experience
-		// Without this, won't be able to find the ASP.NET bits
-		if (process.env['DOTNET_ROOT']) {
-			delete env['DOTNET_ROOT'];
-		}
 		this.execOptions = {
 			env: env
 		};
diff --git a/extensions/notebook/src/jupyter/jupyterSessionManager.ts b/extensions/notebook/src/jupyter/jupyterSessionManager.ts
index c9ec7503b8..e8eee2adea 100644
--- a/extensions/notebook/src/jupyter/jupyterSessionManager.ts
+++ b/extensions/notebook/src/jupyter/jupyterSessionManager.ts
@@ -348,11 +348,6 @@ export class JupyterSession implements nb.ISession {
 			}
 			for (let i = 0; i < Object.keys(process.env).length; i++) {
 				let key = Object.keys(process.env)[i];
-				// DOTNET_ROOT gets set as part of the liveshare experience, but confuses the dotnet interactive kernel
-				// Not setting this environment variable for notebooks removes this issue
-				if (key.toLowerCase() === 'dotnet_root') {
-					continue;
-				}
 				if (key.toLowerCase() === 'path' && this._pythonEnvVarPath) {
 					allCode += `%set_env ${key}=${this._pythonEnvVarPath}${EOL}`;
 				} else {