diff --git a/extensions/sql-migration/src/constants/strings.ts b/extensions/sql-migration/src/constants/strings.ts
index 3ce6db0aec..fdcb69f676 100644
--- a/extensions/sql-migration/src/constants/strings.ts
+++ b/extensions/sql-migration/src/constants/strings.ts
@@ -574,6 +574,8 @@ export function DATABASE_ALREADY_EXISTS_MI(dbName: string, targetName: string):
 	return localize('sql.migration.database.already.exists', "Database '{0}' already exists on the target managed instance '{1}'.", dbName, targetName);
 }
 export const DATABASE_ALREADY_EXISTS_VM_INFO = localize('sql.migration.database.already.exists.vm.info', "Ensure that the provided database name(s) do not already exist on the target SQL Server on Azure Virtual Machine.");
+export const DATABASE_BACKUP_BLOB_FOLDER_STRUCTURE_INFO = localize('sql.migration.blob.storage.folder.info', "When uploading database backups to your blob container, ensure that backup files from different databases are stored in separate folders. Only the root of the container and folders at most one level deep are supported.");
+export const DATABASE_BACKUP_BLOB_FOLDER_STRUCTURE_WARNING = localize('sql.migration.blob.storage.folder.warning', "There are multiple databases with the same backup location selected. Ensure that backup files from different databases are stored in separate folders.");
 export const DATABASE_BACKUP_BLOB_STORAGE_HEADER_TEXT = localize('sql.migration.blob.storage.header.text', "Azure Storage Blob Container details");
 export const DATABASE_BACKUP_BLOB_STORAGE_HELP_TEXT = localize('sql.migration.blob.storage.help.text', "Provide the Azure Storage Blob Container that contains the backups.");
 export const DATABASE_BACKUP_BLOB_STORAGE_TABLE_HELP_TEXT = localize('sql.migration.blob.storage.table.help', "Enter target database name and select resource group, storage account and container for the selected source databases.");
diff --git a/extensions/sql-migration/src/wizard/databaseBackupPage.ts b/extensions/sql-migration/src/wizard/databaseBackupPage.ts
index e933c3b084..f9744c1800 100644
--- a/extensions/sql-migration/src/wizard/databaseBackupPage.ts
+++ b/extensions/sql-migration/src/wizard/databaseBackupPage.ts
@@ -49,6 +49,7 @@ export class DatabaseBackupPage extends MigrationWizardPage {
 	private _blobContainerDropdowns!: azdata.DropDownComponent[];
 	private _blobContainerLastBackupFileDropdowns!: azdata.DropDownComponent[];
 	private _blobContainerFolderDropdowns!: azdata.DropDownComponent[];
+	private _blobContainerFolderStructureInfoBox!: azdata.TextComponent;
 	private _blobContainerVmDatabaseAlreadyExistsInfoBox!: azdata.TextComponent;
 
 	private _networkShareStorageAccountDetails!: azdata.FlexContainer;
@@ -493,6 +494,14 @@ export class DatabaseBackupPage extends MigrationWizardPage {
 				CSSStyles: { ...styles.BODY_CSS }
 			}).component();
 
+		this._blobContainerFolderStructureInfoBox = this._view.modelBuilder.infoBox()
+			.withProps({
+				text: constants.DATABASE_BACKUP_BLOB_FOLDER_STRUCTURE_INFO,
+				style: 'information',
+				width: WIZARD_INPUT_COMPONENT_WIDTH,
+				CSSStyles: { ...styles.BODY_CSS }
+			}).component();
+
 		this._blobContainerVmDatabaseAlreadyExistsInfoBox = this._view.modelBuilder.infoBox()
 			.withProps({
 				text: constants.DATABASE_ALREADY_EXISTS_VM_INFO,
@@ -505,6 +514,7 @@ export class DatabaseBackupPage extends MigrationWizardPage {
 			.withItems([
 				blobTableText,
 				allFieldsRequiredLabel,
+				this._blobContainerFolderStructureInfoBox,
 				this._blobContainerVmDatabaseAlreadyExistsInfoBox,
 				this._blobContainerTargetDatabaseNamesTable])
 			.withProps({ CSSStyles: { 'display': 'none', } })
@@ -711,6 +721,7 @@ export class DatabaseBackupPage extends MigrationWizardPage {
 		await utils.updateControlDisplay(this._networkShareVmDatabaseAlreadyExistsInfoBox, isSqlVmTarget);
 		await utils.updateControlDisplay(this._networkTableContainer, isNetworkShare && !isSqlDbTarget);
 		await utils.updateControlDisplay(this._blobContainer, isBlobContainer && !isSqlDbTarget);
+		await utils.updateControlDisplay(this._blobContainerFolderStructureInfoBox, isBlobContainer && !isSqlDbTarget);
 		await utils.updateControlDisplay(this._blobContainerVmDatabaseAlreadyExistsInfoBox, isSqlVmTarget);
 		await utils.updateControlDisplay(this._blobTableContainer, isBlobContainer && !isSqlDbTarget);
 
@@ -1184,6 +1195,28 @@ export class DatabaseBackupPage extends MigrationWizardPage {
 										if (selectedLastBackupFile && !blobFileErrorStrings.includes(value)) {
 											this.migrationStateModel._databaseBackup.blobs[index].lastBackupFile = selectedLastBackupFile.name;
 										}
+
+										// check for duplicate storage account/blob container/last backup file combination if migrating multiple databases,
+										// as they should all be unique - backups for multiple databases in the same location are not supported
+										let backupLocations: string[] = [];
+										backupLocations = this.migrationStateModel._databaseBackup.blobs.map(blob => {
+											return blob && blob.storageAccount
+												? (blob.storageAccount.id + '/' + utils.getBlobContainerNameWithFolder(blob, this.migrationStateModel._databaseBackup.migrationMode === MigrationMode.OFFLINE)).toLowerCase()
+												: '';
+										}).filter(backupLocation => backupLocation !== '');
+
+										let uniqueBackupLocations = [...new Set(backupLocations)];
+
+										if (uniqueBackupLocations.length !== backupLocations.length) {
+											this.wizard.message = {
+												level: azdata.window.MessageLevel.Warning,
+												text: constants.DATABASE_BACKUP_BLOB_FOLDER_STRUCTURE_WARNING,
+											};
+										} else {
+											this.wizard.message = {
+												text: ''
+											};
+										}
 									}
 								}
 							}));
@@ -1195,6 +1228,28 @@ export class DatabaseBackupPage extends MigrationWizardPage {
 									if (this.migrationStateModel._blobContainerFolders && this.migrationStateModel._blobContainerFolders.includes(value) && !blobFolderErrorStrings.includes(value)) {
 										const selectedFolder = value;
 										this.migrationStateModel._databaseBackup.blobs[index].folderName = selectedFolder;
+
+										// check for duplicate storage account/blob container/folder combination if migrating multiple databases,
+										// as they should all be unique - backups for multiple databases in the same location are not supported
+										let backupLocations: string[] = [];
+										backupLocations = this.migrationStateModel._databaseBackup.blobs.map(blob => {
+											return blob && blob.storageAccount
+												? (blob.storageAccount.id + '/' + utils.getBlobContainerNameWithFolder(blob, this.migrationStateModel._databaseBackup.migrationMode === MigrationMode.OFFLINE)).toLowerCase()
+												: '';
+										}).filter(backupLocation => backupLocation !== '');
+
+										let uniqueBackupLocations = [...new Set(backupLocations)];
+
+										if (uniqueBackupLocations.length !== backupLocations.length) {
+											this.wizard.message = {
+												level: azdata.window.MessageLevel.Warning,
+												text: constants.DATABASE_BACKUP_BLOB_FOLDER_STRUCTURE_WARNING,
+											};
+										} else {
+											this.wizard.message = {
+												text: ''
+											};
+										}
 									}
 								}
 							}));